Our center offers a variety of special methods that can induce conception. While these methods are not absolutely necessary, they can increase the chances of pregnancy by up to a third when combined with optimal treatment. Therefore, it is advisable to consult privately with a doctor before turning to him.
Semen retrieval
In certain cases, it is possible to find no sperm in the ejaculate (Azoospermia) as, for example, in the case of closure of the vas deferens. However, there are alternative methods for obtaining sperm such as:
MASS
Microsurgical aspiration of sperm from the epididymis is a minor operation. In this case, performed under general anesthesia, mature spermatozoa are removed with a micropipette from the epididymal tubes.
FIVE
Percutaneous epididymal sperm aspiration (PESA) is another collection method. A needle biopsy is performed on different layers of testicular tissue and is used in case of obstructive azoospermia.
ory
Testicular sperm biopsy (TESE) is used when sperm is not even present in the testicles. In TESE surgery, performed under general anesthesia, two or more tissue samples are taken, usually from both testicles. If sperm is identified that can be used for assisted fertilization techniques, in most cases the tissue is cryopreserved, then thawed later for ICSI treatment.
TESA
Testicular sperm aspiration (TESA) is a method that involves extracting sperm with a specific needle, performing a percutaneous testicular biopsy. The TESA method is usually used in the presence of obstructive azoospermia.
IMSI (sperm selection)
Morphologically selected intracytoplasmic sperm injection (IMSI) is a special reproductive medicine procedure. In the IMSI method, spermatozoa are selected under a special microscope, with a magnification approximately 6000 times higher than usual. (The equipment used in the ICSI method usually provides only 400 times magnification.) Thanks to this magnification, the internal morphology of the spermatozoa can be recognized. Thus, the IMSI method allows the exclusion of abnormal spermatozoa, which can negatively affect fertilization.
beech
Assisted Hatching is a method that facilitates the exit of the embryo from the zona pellucida. The zona pellucida is made thinner by means of a special laser, which allows the embryologist to create a particularly precise cut on the surface. This method is usually used when the zona pellucida is very thick.
IVM
In Vitro Maturation ("in vitro maturation of oocytes") is an ideal procedure especially for patients who have encountered problems with classical treatments. In the IVM method, previously immature oocytes are left to mature in the incubator. Once the appropriate stage of development is reached, insemination is performed.
ISMs
The supportive placement of the medium in the petri dish where the embryos are located before the embryo transfer represents an opportunity for the optimal preparation of the mucosa for future embryo transfer. Three days after the collection of the oocytes, drops of the culture medium, in which the embryos are located, are inserted into the uterine cavity by means of a catheter. Once the embryo transfer is done, this technique should have a positive effect on the subsequent nest.
Extended cultivation
This technique provides for the extension of embryogenesis until the fifth day; this starts on the day of fertilization of the oocyte until it is transferred to the uterus and in this case the embryo is in the blastocyst stage. Embryos that are transferred at the blastocyst stage have a better chance of conception and pregnancy because they are more mature and the endometrium is more prepared for implantation. Since only about one-third of embryos reach the blastocyst stage, embryo selection allows us to determine which ones have a generally greater potential for development.
Cryopreservation
In cryopreservation, male gametes, female gametes and embryos are stored at -196 ° C in liquid nitrogen, guaranteeing conditions of cell viability.
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