Intracytoplasmic sperm injection (ICSI) is a method of artificial insemination in which, unlike traditional IVF, a sperm is selected and inserted into the oocyte.
Assumptions and therapeutic indications
The ICSI method is the same as the IVF procedure with the essential difference that the spermatozoa are inserted directly into the cytoplasm of the oocytes using a specific instrument (micromanipulator). Thanks to experienced embryologists, in our premises we perform the ICSI method according to the highest standards. In general, we use it as a default procedure, as it allows significantly higher results compared to the traditional IVF method.
Therapeutic indication
The ICSI method is used in the presence of the following diagnoses
- The partner
Obstruction of the ovarian tubes
Psychosomatic disorders
Endometriosis
Polycystic ovary syndrome (PCOs)
- The partner
Reduced concentration of spermatozoa in the seminal fluid
Immobile or poorly motility sperm
Ejaculation dysfunctions
When it is necessary to collect sperm using methods such as: MESA, PESA, TESA, TESE.
treatment
During treatment, the patient is prescribed hormones aimed at hyperovulation. To obtain this result, it is necessary to make injections, usually intramuscular, which can also be performed at home (every day, always at the same time and with the same amount of medication).
The treatment lasts about 10-15 days, depending on the stimulation protocol adopted and how the patient reacts to it. This reaction is monitored with ultrasound scans during the course of treatment.
When the follicles have reached the right size, it is possible to plan the oocyte pick-up.
About 36 hours after receiving the last Gonal-F injection, the oocytes are retrieved from the patient. The reception takes about 10-15 minutes (depending on the number of follicles). After completion, observation is required from 30 minutes to 2 hours to see their maturation (ripening).
On the day of egg collection, it is necessary to collect the partner's sperm by masturbation, as the insemination by means of the ICSI technique takes place immediately afterwards.
If the partner is unable to be present at the clinic on the day when the partner's eggs are collected, the collection of the seminal fluid is foreseen the day before. In this case, cryopreservation (freezing) of the spermatozoa should be performed, which will then be thawed at the moment of insemination by means of the ICSI technique.
3-5 days after insemination using the ICSI technique, the developed embryo is inserted into the uterine cavity using a catheter. After the embryo transfer, the so-called nesting phase begins, where the embryo must be implanted in the uterus. This phase is usually supported by drugs (eg vaginal ovules, injections or even tablets).
About 14 days after the embryo transfer, you should take a pregnancy test (ß-HCG) in your blood or urine to see if you are pregnant or not.
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